Inyathelo elinye RT-qPCR probe kit
Inkcazo
I-One Step qRT-PCR Probe Kit yenzelwe ngokukodwa i-qPCR esebenzisa ngokuthe ngqo i-RNA (umz. i-virus RNA) njengethempleyithi.Ukusebenzisa i-gene specific primers (GSP), i-reverse transcription kunye ne-qPCR inokugqitywa kwityhubhu enye, ukunciphisa kakhulu iinkqubo zemibhobho kunye nomngcipheko wokungcola.Inokuthi ingasebenzi kwi-55 ℃ ngaphandle kokuchaphazela ukusebenza kakuhle kunye novakalelo lwe-qRT-PCR.Inyathelo elinye qRT-PCR Probe Kit ibonelelwe kwi Master Mix.I-5 × One Step Mix iqulethe i-buffer ephuculweyo kunye ne-dNTP/dUTP Mix, kwaye ifanelekile kwiinkqubo zokubona izinto ezikhethekileyo ezisekelwe kwi-fluorescence ebhalwe probes (umz. TaqMan).
Imigaqo esisiseko ye-RT-qPCR
Inkcazo
| Izinto zovavanyo | Inkcazo | Isiphumo |
| (SDS IPHEPHA) Ukucoceka kwesitokhwe se-Enzyme(SDS PAGE) | ≥95% | Dlula |
| Umsebenzi we-Endonuclease | Ayifunyaniswanga | Dlula |
| Umsebenzi we-Exodulease | Ayifunyaniswanga | Dlula |
| Umsebenzi weRnase | Ayifunyaniswanga | Dlula |
| Intsalela ye-E.coli DNA | ＜1 iikopi/60 | Dlula |
| Inkqubo yoVavanyo oluSebenzayo | 90%≤110% | Dlula |
Amacandelo
| Amacandelo | 100rxns | I-1,000rns | 5,000 rxns |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5*umxube wenyathelo elinye | 600μl | 6*1ml | 30ml |
| Inyathelo elinye lomxube we-enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX reference Daye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX isalathiso Idayi 2 | 60μl | 600μl | 3*1ml |
a.Isithinteli esineNqana-linye sibandakanya i-dNTP Mix kunye ne-Mg2+.
b.I-Enzyme Mix iqulethe ikakhulu umva
i-transcriptase, i-Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kunye ne-RNase inhibitor.
c.Isetyenziselwa ukulungisa impazamo yeesinal zefluorescene phakathi kwamaqula ahlukeneyo.
d.I-ROX: Kuya kufuneka ukhethe ulungelelwaniso ngokwemodeli yesixhobo sovavanyo.
Usetyenziso
Ukufunyanwa kwe-QPCR
Ukuthumela ngenqanawa kunye noGcino
Ukuhambisa ngenqanawe:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -20 ℃.
Ubomi beShief:Iinyanga ezili-18
